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Goat Anti-Rabbit IgG H&L antibody (bs-0295G-Cy3)

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說明書： 100ul 1ml

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產品編號	bs-0295G-Cy3
英文名稱	Goat Anti-Rabbit IgG H&L antibody
中文名稱	Cy3標記的羊抗兔IgG H&L
別名	Goat Anti-Rabbit IgG H&L (Cy3); Immunoglobulin G;
	Specific References (72) \| bs-0295G-Cy3 has been referenced in 72 publications. [IF=32.086] Yuxin Liu. et al. Ferromagnetic flexible electronics for brain-wide selective neural recording. ADV MATER. 2022 Nov;:2208251 IF ; Rat. PubMed:36451587 [IF=14.612] Na Zhou. et al. Specific Fluorescent Probe Based on “Protect–Deprotect” To Visualize the Norepinephrine Signaling Pathway and Drug Intervention Tracers. J Am Chem Soc. 2020;142(41):17751–17755 IF ; Human. PubMed:33000941 [IF=7.571] Xiao-Dan Li. et al. Fabricating biodegradable calcium phosphate/calcium sulfate cement reinforced with cellulose: in vitro and in vivo studies. J MATER CHEM B. 2022 Nov;: IF ; Mouse. PubMed:36440610 [IF=7.129] Xinyu Liu. et al. Zearalenone induces oxidative stress and autophagy in goat Sertoli cells. ECOTOX ENVIRON SAFE. 2023 Mar;252:114571 IF ; Goat. PubMed:36708663 [IF=7.129] Shuaiqi Han. et al. Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells. ECOTOX ENVIRON SAFE. 2023 Jul;259:115063 ICC ; Sheep. PubMed:37229875 [IF=6.35] Yue Y et al. Noradrenaline-Specific,?Efficient?Visualization?in?Brain?Tissue?Triggered?by?Unique?Cascade Nucleophilic?Substitution. Anal Chem. 2019 Feb 5;91(3):2255-2259. IHF ; Rabbit. PubMed:30592201 [IF=6.304] Shengbo Chen. et al. Epithelial PBLD attenuates intestinal inflammatory response and improves intestinal barrier function by inhibiting NF-κB signaling. Cell Death Dis. 2021 May;12(6):1-15 IF ; Mouse. PubMed:34059646 [IF=6.17] Wang J et al. Microglial activation contributes to depressive-like behavior in dopamine D3 receptor knockout mice. Brain Behav Immun. 2019 Oct 15. pii: S0889-1591(19)30622-1. IHF ; Rabbit. PubMed:31626970 [IF=6.096] Ying Zhang. et al. Celastrol-based nanomedicine hydrogels eliminate posterior capsule opacification. NANOMEDICINE-UK. 2022 Oct 07 IF ; Rabbit. PubMed:36205091 [IF=6.064] Donglei Liu. et al. Effect of viscoelastic properties of cellulose nanocrystal/collagen hydrogels on chondrocyte behaviors. FRONT BIOENG BIOTECH. 2022; 10: 959409 IHC ; Rat. PubMed:36032700 [IF=6.044] Zhang WN et al. Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb. seeds. Carbohydr Polym. 2019 Apr 1;209:363-371. ICF ; Rabbit. PubMed:30732819 [IF=5.7] Wu X et al. NDP-MSH binding melanocortin-1 receptor ameliorates neuroinflammation and BBB disruption through CREB/Nr4a1/NF-κB pathway after intracerebral hemorrhage in mice. J Neuroinflammation. 2019 Oct 28;16(1):192. IHF-F ; Rabbit. PubMed:31660977 [IF=5.696] Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692 IF ; Human. PubMed:34153644 [IF=5.552] Zhou L et al. Levo-corydalmine Attenuates Vincristine-Induced Neuropathic Pain in Mice by Upregulating the Nrf2/HO-1/CO Pathway to Inhibit Connexin 43 Expression. Neurotherapeutics. 2019 Oct 15. IHF ; Rabbit. PubMed:31617070 [IF=5.38] Zhuang, Likun, et al. "MicroRNA-23b functions as an oncogene and activates AKT/GSK3β/β-catenin signaling by targeting ST7L in hepatocellular carcinoma." Cell Death & Disease 8.5 (2017): e2804. IF(ICC) ; Rabbit. PubMed:28518144 [IF=5.201] Cao Jun. et al. Propofol Exposure Disturbs the Differentiation of Rodent Neural Stem Cells via an miR-124-3p/Sp1/Cdkn1b Axis. Front Cell Dev Biol. 2020 Aug;8:838 ICC ; Rat. PubMed:32984332 [IF=5.192] Anmei Shu. et al. Catalpol ameliorates endothelial dysfunction and inflammation in diabetic nephropathy via suppression of RAGE/RhoA/ROCK signaling pathway. Chem-Biol Interact. 2021 Aug;:109625 IF ; Mouse. PubMed:34416245 [IF=5.135] Wei Lu. et al. Effects of targeted muscle reinnervation on spinal cord motor neurons in rats following tibial nerve transection. Neural Regen Res. 2022 Jan;17(8):1827 IF ; Rat. PubMed:35017445 [IF=5.08] Imamura, Tadatsugu, et al. "Antigenic and receptor binding properties of Enterovirus 68." Journal of Virology (2013): JVI-03070. other ; Rabbit. PubMed:24371050 [IF=5.076] Cao Jun. et al. Supplemental N-3 Polyunsaturated Fatty Acids Limit A1-Specific Astrocyte Polarization via Attenuating Mitochondrial Dysfunction in Ischemic Stroke in Mice. Oxid Med Cell Longev. 2021;2021:5524705 IF ; Mouse. PubMed:34211624 [IF=5.076] Feng T et al. Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis PathwayOxid Med Cell Longev.2020 Dec 31;2020:5954635. IF ; Goat. PubMed:33488926 [IF=5.008] Liu, W., et al. "PAX3 is a novel tumor suppressor by regulating the activities of major signaling pathways and transcription factor FOXO3a in thyroid cancer." Oncotarget (2016). IF(ICC) ; Rabbit. PubMed:27458157 [IF=5.008] Shi et al. Frequent amplification of AIB1, a critical oncogene modulating major signaling pathways, is associated with poor survival in gastric cancer. (2015) Oncotarget. 6:14344-59 IF ; Rabbit. PubMed:25970779 [IF=4.85] Shaoqiu Wu. et al. GPX2 stabilized by PCBP2 induces autophagy to protect Het-1A esophageal cells from apoptosis and inflammation. CELL SIGNAL. 2022 Sep;97:110397 IF ; Human. PubMed:35798180 [IF=4.658] Gong Q et al. Enhanced?ROBO4?is?mediated?by?up-regulation?of HIF-1α/SP1?or?reduction?in?miR-125b-5p/miR-146a-5p?in?diabetic?retinopathy. J Cell Mol Med.?2019 Jul;23(7):4723-4737. ICF ; Rabbit. PubMed:31094072 [IF=4.57] Liu, Hongxia, et al. "Ultrasound-Mediated Destruction of LHRHa Targeted and Paclitaxel Loaded Lipid Microbubbles Induces Proliferation Inhibition and Apoptosis in Ovarian Cancer Cells." Molecular Pharmaceutics (2013). ICF ; Rabbit. PubMed:24266423 [IF=4.389] Jiuxin Zhu. et al. GPC3-targeted and curcumin-loaded phospholipid microbubbles for sono-photodynamic therapy in liver cancer cells. Colloid Surface B. 2021 Jan;197:111358 IHC ; Human. PubMed:33068823 [IF=4.379] Ouyang, Xin. et al. Ketamine ameliorates depressive-like behaviors in mice through increasing glucose uptake regulated by the ERK/GLUT3 signaling pathway. Sci Rep-Uk. 2021 Sep;11(1):1-13 IF ; Mouse. PubMed:10.1038/s41598-021-97758-7 [IF=4.113] Fengyang Li. et al. Pretreatment of Indobufen and Aspirin and their Combinations with Clopidogrel or Ticagrelor Alleviates Inflammasome Mediated Pyroptosis Via Inhibiting NF-κB/NLRP3 Pathway in Ischemic Stroke. J Neuroimmune Pharm. 2021 Jan 29 IF ; Rat. PubMed:33512659 [IF=4.102] Huaming Xi. et al. FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells. Mol Cell Endocrinol. 2021 Nov;:111505 IF ; Goat. PubMed:34774699
抗體來源	Goat
克隆類型	Polyclonal
交叉反應	Human,Mouse,Rat,Rabbit
產品應用	Flow-Cyt=1:50-200,IF=1:200-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
性狀	Liquid
濃度	2.0 mg/ml
免疫原	Native rabbit IgG
亞型	IgG
純化方法	affinity purified by Protein G, nonspecific adsorbed
緩沖液	10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存條件	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹	Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
產品圖片	Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with LRP2 Monoclonal Antibody, Unconjugated (bsm-42103R) at 1:200 overnight at 4°C, followed by a Cy3 conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-Cy3) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (c-Maf) Polyclonal Antibody, Unconjugated (bs-5977R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G- cy3) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (c-Maf) Polyclonal Antibody, Unconjugated (bs-5977R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G- cy3) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (c-Maf) Polyclonal Antibody, Unconjugated (bs-5977R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G- cy3) for 90 minutes, and DAPI for nuclei staining.

	1、抗體溶解方法
	2、抗體修復方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關于肽鏈的設計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序

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